Bio agent code assay strip

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After 15—20 minutes, the pH was assessed and adjusted if needed with minimal volume change to 7. In the present study, HHAs from multiple manufacturers were evaluated for their effectiveness at detecting two specific targets, SEB and ricin, in multiple water matrices. For chlorine-treated SEB, no band was visible at the same molecular weight as untreated SEB for any of the time points tested. View at Google Scholar B. The percentages reported here were obtained using the Reed reference set for the secondary structure calculations [ 9 ].

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Additionally, HHAs have proven fast and effective in the laboratory for the detection of Francisella tularensis [ 1 ] and biotoxins such as staphylococcal enterotoxin B SEB [ 2 ] and microcystins [ 3 ].

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International Journal of Microbiology

These results support the idea that increased LODs in disinfected water are caused by disruption in antigen-antibody binding due to degradation of the antigen. After 24 hours, sodium thiosulfate 0. SEB could potentially be used to attack low-volume water supplies; however, due to the fact that ricin is less toxic by oral ingestion, it may be a less likely threat to drinking water [ 10 ]. Detection with HHAs was performed immediately after spiking each water with toxin. However, regardless of whether activity remains, the ability to detect the presence of each toxin in disinfected water supplies is important. Abstract Development of a rapid field test is needed capable of determining if field supplies of water are safe to drink by the warfighter during a military operation. In fact, sodium hypochlorite is known to completely inactivate SEB and ricin toxin [ 1112 ].

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